Bacterial

reassessment summer 2009

MS1005N General Biochemistry (Module Leader: Dr D. Perumal)
Summary

ComponentReassessment TaskReassessment Environment (include length if exam)Additional info
(optional)
CWK realistic ReportNon time-constrained Practical Report 1000 words turn over in by standard deadline
EXU spiritual world ExamUnseen time-constrained examinationExam
(1 hour)
OTH Practical AttendanceNot reassessedn/a

Coursework Practical Report based on Practical 2:

SEPARATION OF ANALGESICS BY THIN LAYER ADSORPTION CHROMATOGRAPHY

instruction manual:

?The practical brief for Practical 2 is as on Page 18 of the 2008-09 Module Booklet. It is also included below.

?You ar required to write a full practical musical composition (1000 words) using the results obtained during the practical. Please read the guidelines at the end of this agenda when writing up.

Practical 2
Introduction

Separation of compounds on a cut down layer of supporting medium (i.e. TLC or frail layer chromatography) is very rapid and gives good resolution at low concentrations. Separation whitethorn be by adsorption, ion exchange, division or gelatin filtration depending on the medium employed.



Separated compounds may be detected by methods not possible on constitution, for example with corrosive sprays; in this practical the isolated spy ar visualised under ultraviolet (UV) light. Here analgesics are separated by adsorption on a contract layer of silica gel using an ethyl ethanoate/ethanol/ethanoic acid solvent mixture.

Method

TLC plates

Silica gel TLC aluminium sheets will be provided. Handle them cautiously by their edges to prevent flaking.

Preparation of chromatography tank

Line small beakers with paper which as it becomes soaked in the solvent produces a stark(a) atmosphere. Add the solvent provided to the beaker to a depth of about 1 cm and cover with clingfilm.Beware of letting the silica come into encounter with the solvent-saturated paper.

Chromatography

The...If you want to get a full essay, order it on our website: Orderessay



If you want to get a full essay, wisit our page: write my essay .