Total Number of Yeast

TITLE :
Dilution of barm suspension over the total event of barm cells per cm3.
AIM :
To determine the total outlet of yeast cells per cm3 in dissimilar dilutions of yeast suspension.
RESEARCH QUESTION :
Does the different dilution of yeast suspension affect the total number of yeast cells per cm3?
INTRODUCTION :
Yeast is a fungus, which under certain conditions produces alcohol and carbon dioxide from sugars, by fermentation .Counting of yeast cells through microscope disregard be obtained by using the haemocytometer in different dilution of yeast suspension.
HYPOTHESIS :
The higher the dilution of yeast suspension,the lower the total number of yeast cells seen by the microscope.This is because the increase in volume of distilled water makes the suspension of yeast more dilute.Hence,the number of yeast cells counted conk lesser.
DEPENDENT VARIABLE : The total number of yeast cells seen by the microscope.
INDEPENDENT VARIABLE : The dilution of yeast suspension.
CONSTANT VARIABLE: The subject of yeast suspension,volume of distilled water in each test metro.




setup AND MATERIAL :
APPARATUSQUANTITY (s)
Haemocytometer and coverslip1
Tissue paper1
lens system tissue1
Dropper1
Petri dish with moist tissue1
Microscope1
fleet tally counter1


MATERIALQUANTITY (s)
Alcohol some drops
Distilled water50 ml
Suspension of yeast cells5 ml

number :
A.Setting up the lantern slide
1.The haemocytometer is cleaned with alcohol.
2.The haemocytometer is then wiped by using the lens tissue.
3.The slide is moistened by using a damp tissue.
4.A special coverslip is pushed on the slide and it is then pressed down on the extraneous edges of the coverslip at the same time until a Newtons rings is seen.

B.Loading the haemocytometer
5.The yeast suspension is shaked gently.
6.The end of the capillary electron tube is inserted into the suspension.The liquid will rise into the tube.
7.The end of the capillary tube is then run along the edge of the coverslip between...If you want to fit a full essay, order it on our website: Orderessay



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